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上海奥源生物科技有限公司 ꄲ 产品中心 ꄲ 细胞培养 ꄲ 核酸提取 ꄲ AW-58120 AW-58120S LS Reagent Total RNA Extraction Reagent in Liquid Sample

AW-12030DMEM,HighGlucose,noglutamine,nophenolredDMEM高糖不含L-谷氨酰胺，不含丙酮酸钠，不含酚红

ꁆ ꁇ

AW-58120 AW-58120S LS Reagent Total RNA Extraction Reagent in Liquid Sample

售价：

¥ 1125.00

原价： ¥1500.00

ꄑ关注：0

服务：･ 30天无忧售后･专业技术辅导实验･专人专送免邮･奥源自营

100ML/200ML: 200ML

100ML

数量： ꄷ ꄸ

Product: LS Reagent Total RNA Extraction Reagent in Liquid Sample

Catalog Number		Product Size
AW-58120		100ML
AW-58120S		200ML
Downstream Application		Cloning, Northern Blotting,
		Nuclease Protection Assays,
		Real-Time, Quantitative PCR(qPCR),
		Reverse Transcriptase PCR (RT-PCR),
		cDNA Library Construction
Final Product		Total RNA, Transcriptome RNA,
		micro RNA
High Throughput Compatibility		Not High
		Throughput-Compatible (Manual)
Isolation Technology		Organic Extraction
Number of Reaction		200Preps
Purification Time		1 hr
Sample Type (General)		Blood, Liquid Samples (e.g. Serum),Viral Samples
Scale		Scalable
Shipping Condition		Room Temperature
Storage conditions		Room Temperature(protect from light)
Shelf life		24months from date of manufacture
Starting Material (Amount)		250 µl to 1 ml liquid samples (suggested range; scalable)
Yield (up to)		8 mg
TEST	SPECIFICATION		RESULT
Appearance	Clear reddish solution
Absorbance (260/280) of RNA	1.7-2.0		Meets specification
Agarose gel electrophoresis	Shows most of the isolated DNA in close proximity to the application slots (60 - 100 kb) and 20% of DNA migrating as a band of size 20 kb.		Meets specification
RT-PCR	Analysis shows RNA integrity is appropriate for reverse transcription and amplification of cDNA.		Meets specification
Bioanalyzer	Two intact ribosomal bands with 2:1 ratio of large to small ribosomal RNA.		Meets specification
DNA Digestion	Incubation with restriction endonuclease results in digestion of more than 80% of DNA.		Meets specification
Instructions: This product can only be used for scientific research or further production, not for medical diagnosis.

ALLWIN LS reagent is a reagent that directly extracts total RNA from liquid samples derived from people, plants and animals, yeast, bacteria and viruses. It can also extract total RNA from animal tissues, plant materials, various microorganisms and cultured cells. This method has a good separation effect on a small number of tissues (50-100mg) and cells (5x106) as well as a large number of tissues (≧1g) and cells (>1×107). The sample is fully cleaved in LS to maximize RNA integrity. After chloroform was added for centrifugation, the solution was divided into three layers: the upper colorless aqueous phase, the middle layer, and the lower red organic phase. RNA was distributed in the supernatant layer. After collecting the supernatant, total RNA can be recovered by isopropanol precipitation. The extracted total RNA had good integrity and was free of protein and DNA contamination, which could be used in various molecular biology routine experiments, such as RT-PCR, Real-time RT PCR, Northern Blot, Dot Blot, In vitro Translation, etc.

ALLWIN LS reagent can promote the precipitation of various RNA of different species and different molecular weight sizes. For example, RNA agarose gel electrophoresis from rat liver and ethyl bromide staining showed many discontinuous stripe of high molecular weight (mRNA and hnRNA components) between 7kb and 15kb, two dominant ribosomes -5kb (28S) -2kb (18S), and low molecular weight =RNA between 0.1 and 0.3 kb(tRNA, 5S). When the extracted RNA was diluted with TE, its A260/A280 ratio was 1.8. (note that if agarose gel electrophoresis is used, the position of 28S is about 2kb, and the position of 18S is about 1kb. The gel position varies greatly with different concentrations.)

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